Characterization of a Chromosomally Encoded 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase from Burkholderia sp. Strain RASC
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Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC.
The findings of previous studies indicate that the genes required for metabolism of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-D) are typically encoded on broad-host-range plasmids. However, characterization of plasmid-cured strains of Burkholderia sp. strain RASC, as well as mutants obtained by transposon mutagenesis, suggested that the 2,4-D catabolic genes were located on the chromoso...
متن کاملEvidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/(alpha)-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp.
We characterized the gene required to initiate the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) by the soil bacterium Burkholderia sp. strain TFD6, which hybridized to the tfdA gene of the canonical 2,4-D catabolic plasmid pJP4 under low-stringency conditions. Cleavage of the ether bond of 2,4-D by cell extracts of TFD6 proceeded by an (alpha)-ketoglutarate-dependent reaction, characte...
متن کاملEvidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp
Evidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp. V. GRACE MATHESON, LARRY J. FORNEY,* YUICHI SUWA,† CINDY H. NAKATSU,‡ ALAN J. SEXSTONE, AND WILLIAM E. HOLBEN§ Department of Plant Pathology and Environmental Microbiology, West Virginia University, Morgantown, West Virginia 26506, and Center for Mic...
متن کاملCapture of a catabolic plasmid that encodes only 2,4-dichlorophenoxyacetic acid:alpha-ketoglutaric acid dioxygenase (TfdA) by genetic complementation.
The modular pathway for the metabolism of 2,4-dichlorophenoxyacetic acid (2,4-D) encoded on plasmid pJP4 of Alcaligenes eutrophus JMP134 appears to be an example in which two genes, tfdA and tfdB, have been recruited during the evolution of a catabolic pathway. The products of these genes act to convert 2,4-D to a chloro-substituted catechol that can be further metabolized by enzymes of a modif...
متن کاملPartial Purification and Characterization of 2,4- Dichlorophenoxyacetic Acid Degrading Bacteria Harboring Alpha Ketoglutarate Dioxgenase Enzyme
The catabolic adaptability demonstrated by microorganisms is important in the bioremediation of polluted environments. 2,4-dichlorophenoxyacetic acid/α-ketoglutarate dioxygenase is the first enzyme in the catabolic pathway for the 2,4dichlorophenoxyacetic acid (2,4-D). In the present study, dioxygenase enzymes were inducible and the serial adaptation of four studied bacterial isolates steadily ...
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تاریخ انتشار 2016